ABSTRACT
Objective:To investigate the tissue distribution of asiaticoside in SD rats .Methods:The rats were injected by asiatico-side at the effective treatment dose of 42 mg kg -1 via tail vein.The rats sacrificed respectively at 5, 30 and 80 min after the injection and heart, liver, spleen, lung, kidney, stomach, intestine, brain, gonad and left thigh muscle were dissected immediately .The con-tent of asiaticoside in the tissues was determined by HPLC .Results:Asiaticoside widely distributed in SD rats , and the concentration in heart was the highest followed by liver , brain, lung, spleen and kidney .The contents in the other tissues were relatively low .Con-clusion:The method is convenient , sensitive and accurate , and can be used for the content determination of asiaticoside the tissues of SD rats.
ABSTRACT
Objective:To establish a method for madecassoside determination by solid-phase extraction RP-HPLC in SD rats plasma. Methods:The sample was cleaned with an Strata-X solid-phase extraction . HPLC conditions were as follows:Welchrom-C18 column (250 mm?4.6 mm,5 ?m),water-acetonitrile(24∶76)as mobile phase(flowrate:1.0 ml/min),cucurbitacin B as internal standard, detection wavelength of 225 nm,and column temperature of 30℃. Results:In this situation,the remaining times of the madecassoside and cucurbitacin B were 5.11 min and 8.14 min,respectively. The linear range of the method was 5 ?g/ml～1.5 mg/ml (r=0.999 8).The low limit of detection was 1 ?g/ml. The method recoveries and the extraction recoveries were 90.21%～95.65% and 80.65%～87.04%,respectively. The within-day RSD and between-day RSD were between 1.31%～3.07%(n=5). Conclusion:Themethod is simple,convenient,accurate, reproducible,and suitable for studying the pharmacokinetics of madecassoside in SD rats .